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目的 研究转化生长因子 (TGF) β1、碱性成纤维细胞生长因子 (b FGF)、白细胞介素 8(IL 8)、白细胞介素 13(IL 13)、γ干扰素 (IFN γ)在普通型间质性肺炎 (UIP)和非特异性间质性肺炎(NSIP)肺组织中的分布、表达及意义。方法 经胸腔镜或开胸肺活检获取 5例UIP和 8例NSIP患者的肺组织。对照组 5例 ,来自手术切除的远离肺癌原发灶的周边肺组织。用免疫组化法半定量分析细胞因子的分布及表达。结果 TGF β1、IL 8、b FGF主要分布在肺泡上皮细胞、肺泡巨噬细胞、细支气管上皮细胞 ,UIP组表达强于NSIP组和对照组。IL 13主要分布在肺泡上皮细胞、肺泡巨噬细胞、间质单个核细胞 ,UIP、NSIP组表达无明显差异 ,但均强于对照组。IFN γ主要分布在间质单个核细胞 ,NSIP组表达强于UIP组和对照组。UIP组的IL 13/IFN γ比值为 (2 18± 0 76 ) ,NSIP组为(0 95± 0 2 8) ,对照组为 (0 91± 0 16 ) ,3组比较差异均有显著性 (P值均 <0 0 5 ) ,而NSIP组与对照组比较差异无显著性。对照组只有肺泡巨噬细胞表达上述各细胞因子。结论 TGF β1、IL 8、b FGF在UIP和NSIP患者肺组织中表达强度的不同和IL 13/IFN γ的是否平衡可能参与了UIP和NSIP不同的发病过程。
Objective To investigate the effects of transforming growth factor β1 (TGF β1), basic fibroblast growth factor (b FGF), interleukin 8 (IL 8), interleukin 13 (IL 13) and interferon gamma Interstitial pneumonia (UIP) and non-specific interstitial pneumonia (NSIP) lung tissue distribution, expression and significance. Methods Thoracoscopy or open lung biopsy was performed to obtain the lung tissues of 5 UIP patients and 8 NSIP patients. Control group, 5 cases, from the surgical excision of the lung far from the primary lung cancer. Semi-quantitative analysis of the distribution and expression of cytokines by immunohistochemistry. Results TGF β1, IL 8 and b FGF were mainly distributed in alveolar epithelial cells, alveolar macrophages and bronchiolar epithelial cells. The expressions of TGFβ1, IL 8 and bFGF were stronger in the UIP group than in the NSIP group and the control group. IL 13 mainly distributed in alveolar epithelial cells, alveolar macrophages, interstitial mononuclear cells, UIP, NSIP group showed no significant difference, but were stronger than the control group. IFNγ mainly distributed in interstitial mononuclear cells, NSIP group was stronger than UIP group and control group. The IL13 / IFN γ ratio was (2 18 ± 0 76) in the UIP group, (0 95 ± 0 28) in the NSIP group and (0 91 ± 0 16) in the control group, with significant differences among the 3 groups P <0.05), while there was no significant difference between NSIP group and control group. Only alveolar macrophages in the control group expressed the above cytokines. Conclusion The different expression of TGF-β1, IL-8 and b-FGF in the lung tissue of UIP and NSIP patients and the balance of IL-13 / IFN-γ may be involved in the different pathogenesis of UIP and NSIP.