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用Inter-简单重复序列(ISSR)标记对樟子松东北分布区9个种源的135 个个体遗传变异进行了检测。10 个ISSR 引物扩增共检测到108 个位点,其中,多态位点百分率差异明显,范围在27%～54%之间,多态位点百分率最高的是红花尔基,最低的是卡伦山。物种水平的Shannon 多样性指数(I)为0.1581,Nei 基因多样度(h)为0.2393。遗传变异在种源间占39.65%,在种源内占60.35%。根据参试樟子松9 个种源的聚类图,结果樟子松自然分布区划为2 个种源区:大、小兴安岭种源区;呼伦贝尔种源区。图2 表3 参18。
The genetic variation of 135 individuals from 9 provenances in the northeast of Pinus sylvestris var. Mongolica was detected by Inter-simple repeat (ISSR) markers. A total of 108 ISSR primers were detected by PCR amplification, among which, the percentages of polymorphic loci were significantly different, ranging from 27% to 54%. The highest percentage of polymorphic loci was Honghuaerji, the lowest was Karen Hill. Shannon’s diversity index (I) at the species level was 0.1581, and the Nei gene diversity (h) was 0.2393. Genetic variation accounted for 39.65% among provenances and 60.35% within provenances. According to the cluster map of nine provenances of Pinus sylvestris var. Mongolica, the natural distribution of Pinus sylvestris var. Mongolica is divided into two provenance areas: the germplasm area of Xiaoxing’an Mountains and the provenance of Hulun Buir. Figure 2 Table 3 Reference 18.