论文部分内容阅读
采用来自广东各稻作区的39个不同致病型稻瘟病代表菌株,对由沪旱B经空间突变而来的优质籼稻抗源DH104进行抗谱分析.结果发现,DH104的抗谱达到100%,而8个单基因品系的抗谱均在80%以下.为了挖掘和定位DH104含有的稻瘟病抗性基因,选用广致病谱病菌GD0193对DH104进行遗传分析.用GD0193接种DH104和高感稻瘟病籼稻材料L101杂交获得的F2群体,结果发现,F2群体抗病与感病植株的比例符合3∶1的理论比值(313R∶97S,2=0.896 1),表明DH104对GD0193的抗性由一个显性主效基因控制,暂命名为Pixt(t)基因.利用SSR标记将其初步定位在第6染色体短臂RM136和RM7213之间,遗传距离分别为4.9和1.5 cM.
By using the representative strains of 39 pathogenic rice blast isolates from various paddy districts in Guangdong, the antihyperinophores analysis of high quality indica rice DH104 derived from space mutation of Shanghai drought and drought B showed that the resistance spectrum of DH104 reached 100% , While the resistance spectrum of 8 single-line strains was less than 80% .In order to excavate and locate the blast resistance gene contained in DH104, genetic analysis of DH104 was carried out using GD0193, The results showed that the proportion of disease-resistant and susceptible plants in F2 population was in accordance with the theoretical ratio of 3:1 (313R: 97S, 2 = 0.896 1), indicating that the resistance of DH104 to GD0193 was caused by An dominant dominant gene, tentatively named Pixt (t), was initially mapped on the short arm of chromosome 6 between RM136 and RM7213 using SSR markers with a genetic distance of 4.9 and 1.5 cM, respectively.