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目的在检测不同剂量碘-131对小鼠胸腺细胞自发掺入3H-TdR的基础上,观察碘-131对小鼠体外胸腺细胞自发掺入3H-TdR的影响。方法给予不同剂量碘-131组的小鼠胸腺细胞同体积的3H-TdR,采用3H-TdR自发掺入法检测各组胸腺细胞每分钟计数率(CPM)。结果终浓度为5 Bq/ml的碘-131组小鼠胸腺细胞自发掺入3H-TdR的CPM值显著高于正常胸腺细胞,终浓度为5×105Bq/ml和5×106Bq/ml组小鼠胸腺细胞自发掺入3H-TdR的CPM值明显低于正常胸腺细胞。结论低剂量的碘-131对小鼠胸腺细胞自发掺入3H-TdR具有显著的刺激作用,高剂量的碘-131对小鼠胸腺细胞自发掺入3H-TdR有明显的抑制作用。
Objective To detect the effects of iodine-131 on the spontaneous incorporation of 3H-TdR into thymocytes of mice induced by different dosages of iodine-131 on spontaneous incorporation of 3H-TdR into mouse thymocytes. Methods The same volume of 3H-TdR of mouse thymocytes in different doses of iodine-131 group was given. The thymocyte counts per minute (CPM) of each group were detected by 3H-TdR spontaneous incorporation method. Results The CPM value of spontaneous incorporation of 3H-TdR in iodine-131 group mice with a final concentration of 5 Bq / ml was significantly higher than that of normal thymocytes, with a final concentration of 5 × 10 5 Bq / ml and 5 × 10 6 Bq / ml The thymocyte spontaneous incorporation of 3H-TdR CPM value was significantly lower than normal thymocytes. Conclusion Low dose iodine-131 can stimulate 3H-TdR incorporation spontaneously in mouse thymocytes. High dose iodine-131 can significantly inhibit 3H-TdR incorporation into mouse thymocytes.