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作者采用生物素-亲和素系统,建立了测定人血清多聚白蛋白受体(PHSA-R)的生物素-亲和素ELISA技术,并探讨了一些影响因素及临床意义,现摘要报道如下。 一、材料 人血清白蛋白,上海生物制品研究所提供.按Len-kei等方法制备人血清多聚白蛋白(PHSA),聚丙烯酰胺凝胶电泳证明为多聚体。生物素化抗-HBs(抗-
The authors have established a biotin-avidin ELISA for the determination of human serum albumin receptor (PHSA-R) using biotin-avidin system and discussed some influential factors and clinical implications. The abstract is reported as follows . Materials Human serum albumin, provided by Shanghai Institute of Biological Products, prepared by the method of Len-kei human serum albumin (PHSA), polyacrylamide gel electrophoresis proved to be multimers. Biotinylated anti-HBs (anti-