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为了全面探讨水飞蓟素对心血管系统的作用,观察了水飞蓟素和水飞蓟宾对大鼠血小板聚集和粘附功能的影响。 实验用Wistar大鼠,使用PAM-2型PPP自动平衡血小板聚集仪,按文献报道方法测定血小板聚集反应。分别以2.75％水飞蓟素10μl和6.57％水飞蓟宾5μl加入0.2ml的富血小板血浆中预孵5min,然后以ADP(0.2 mmol/L,10μl)诱导聚集。对照以等体积对照液加入PRP中。 大鼠分别静脉注射水飞蓟紊(80mg/kg)和水飞蓟宾(60mg/kg),分别于给药前后1h测定血小板粘附率。 结果:水飞蓟素和水飞蓟宾使大鼠血小板最大聚集率分别降低33％和68％,与对照比较P<0.01。水飞蓟素和水飞蓟宾均显著降低大鼠血小板粘附率,给药前后比较P<0.01。
In order to comprehensively investigate the effects of silymarin on the cardiovascular system, the effects of silymarin and silybin on platelet aggregation and adhesion function in rats were observed. Experimental Wistar rats, PAM-2-type PPP automatically balance platelet aggregation device, according to the literature method for determination of platelet aggregation reaction. To 0.2 ml platelet-rich plasma, 5 μl of 2.75% silymarin and 5 μl of 6.57% silybin were separately preincubated for 5 min and then induced to aggregate with ADP (0.2 mmol / L, 10 μl). Control was added to PRP as an equal volume of control. Silymarin (80mg / kg) and silybin (60mg / kg) were intravenously injected into rats, and the rate of platelet adhesion was measured at 1 hour before and after administration. Results: Silymarin and silybin significantly reduced the maximum platelet aggregation in rats by 33% and 68%, respectively, compared with the control (P <0.01). Silymarin and silybin significantly reduced the rate of platelet adhesion in rats, P <0.01 before and after administration.