论文部分内容阅读
目的 探讨超抗原(SAg)金黄色葡萄球菌肠毒素A(SEA)与抗人胃癌细胞单克隆抗体Fab’偶联蛋白的抗肿瘤活性。方法 利用SEA-Fab’激活效应细胞诱导的靶细胞凋亡指数检测。分别在靶细胞Walker-256加入SEA-Fab’、SEA,另设PBMC+Walker-256细胞作为对照,作用时间分别为8、36和72h,观察该偶联蛋白的超抗原活性和激活T细胞杀伤肿瘤细胞的作用。结果 凋亡指数检测各处理组之间差异有非常显著性(P<0.01)。其中,除了SEA-Fab’组与SEA组与效应细胞+靶细胞组之间差异有非常显著性(P<0.01),SEA-Fab’组与SEA组差异有非常显著性(P<0.01)。结论 SEA-Fab’偶联蛋白比单独SEA能更有效地激活T细胞的杀伤肿瘤细胞活性,以单克隆抗体为载体进行肿瘤导向治疗具有可行性。
Objective To investigate the antitumor activity of superantigen (SAg) Staphylococcal enterotoxin A (SEA) and anti-human gastric cancer cell monoclonal antibody Fab ’-conjugated protein. Methods The apoptosis index of target cells induced by SEA-Fab ’activating effector cells was detected. SEA-Fab ’, SEA and PBMC + Walker-256 cells were added to the target cells as controls respectively for 8, 36 and 72 h, respectively. The superantigen activity and activated T cell killing activity of the coupled proteins were observed The role of tumor cells. Results There was a significant difference in apoptotic index between treatment groups (P <0.01). Among them, the difference between SEA-Fab ’group and SEA group and effector cell + target cell group was significant (P <0.01), SEA-Fab’ group was significantly different from SEA group (P <0.01). Conclusions SEA-Fab’conjugated protein can activate T cell cytotoxic activity more effectively than SEA alone. It is feasible to use monoclonal antibody as carrier for tumor therapy.