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1996～1997年用诊断剂量法监测,证明冀鲁豫6个棉铃虫种群对三氟氯氰菊酯、氯氰菊酯、灭多威、久效磷和辛硫磷已有较高的抗性频率。其中33％的样本抗性频率在50％以上,51％的样本抗性频率在20％～50％之间,只有16％的样本抗性频率在20％以下。点滴法测定结果表明,棉铃虫对三氟氯氰菊酯的抗性指数(RR)在47.8倍以上,最高达195.6倍。对灭多威抗性达12.6～132.7倍,对久效磷抗性为9.2～26.5倍。浸叶法与诊断剂量法和点滴法结果基求一致。增效醚(PBO)对三氟氯氰菊酯的增效作用显著大于三丁基磷酸三硫酯(DEF),表明多功能氧化酶是其抗性的主要解毒酶。点滴法监测新疆棉铃虫对农药的抗性基本在10倍以下,浸叶法均在1倍以下。最后讨论了如何设计和实施有效的抗性治理方案。
From 1996 to 1997, it was proved by diagnostic dosimetry that six cotton bollworm populations in Hebei and Luzhou had higher frequency of resistance to cyhalothrin, cypermethrin, methomyl, monocrotophos and phoxim. Among them, 33% of the samples have a frequency of over 50%, 51% of the samples have a frequency of between 20% and 50%, and only 16% of the samples have a frequency below 20%. The results of drip method showed that the resistance index (HR) of Helicoverpa armigera to cyhalothrin was above 47.8 times, up to 195.6 times. Resistance to methomyl was 12.6 to 132.7 times, and to monocrotophos was 9.2 to 26.5 times. Leaf dipping method and the diagnostic dose method and drip method based on the same results. The synergistic effect of PBO on cyhalothrin was significantly greater than that of tridecyl tributyl phosphate (DEF), indicating that multifunctional oxidase is the major detoxifying enzyme for its resistance. Point-by-point method to monitor Xinjiang cotton bollworm resistance to pesticides in the basic 10 times less, soaking leaf method are less than 1 times. Finally, we discuss how to design and implement effective resistance management programs.