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目的了解满洲里口岸地区医学媒介生物及其携带病原情况。方法 2013年8月,在满洲里口岸地区采用单公顷一日弓形夹法、5 m夹线法捕获鼠类,将捕获的鼠类麻醉后检蚤、蜱;采用诱蝇笼诱捕法捕获蝇类;采用诱捕法捕获蜚蠊。对捕获的媒介生物进行分类鉴定,利用核酸检测技术及细菌分离培养技术进行病原体检测。结果捕获鼠类4种18只、蜱类1种19只、蝇类6种675只、蜚蠊1种80只。从2份鼠类样本中检测到支原体特异性核酸片段;从8份蜱类样本中检测到斑点热群立克次体特异性核酸片段;从675份蝇类样本中共分离到16种致病菌(条件致病菌);从80份蜚蠊样本中分离到13种致病菌(条件致病菌)。结论蝇类16种致病菌和蜚蠊13种致病菌均为满洲里口岸首次检出,应进一步加强对满洲里口岸地区医学媒介生物的监测,以保障满洲里口岸的卫生安全。
Objective To understand the status of medical vectors and pathogenic carriers in Manzhouli Port. Methods In August 2013, a single hectare bow of a tree was used in Manchuria port area to capture rodents by the 5 m line method. The captured rodents were anesthetized and fleas and ticks were seized. Trap flies were captured by fly trap catches. Trapping cockroaches by trapping method. Identification of the captured vector organisms, the use of nucleic acid detection technology and bacterial isolation and culture technology for pathogen detection. Results There were 18 species of 4 species, 19 species of ticks, 6 species of 6 species of flies and 80 species of 1 species of cockroaches. Mycoplasma-specific nucleic acid fragments were detected from 2 murine samples; spotted heat group Rickettsia specific nucleic acid fragments were detected from 8 ticks; 16 kinds of pathogenic bacteria were isolated from 675 flies samples (Opportunistic bacteria). Thirteen pathogenic bacteria (opportunistic bacteria) were isolated from 80 samples of cockroach. Conclusion The 16 pathogenic bacteria of flies and 13 pathogens of cockroaches were detected for the first time in Manzhouli port. The monitoring of medical vector organisms in the port area of Manzhouli should be further strengthened so as to ensure the hygiene and safety of the ports in Manchuria.