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目的:通过两种不同中药方剂温脾补肾法(方剂1)和理气化痰降浊法(方剂2)经验方对阿尔茨海默病(AD)APP/PS1转基因小鼠学习记忆能力影响的对照研究探讨两首中药方剂治疗AD的可能性。方法:24只AD小鼠随机分为对照组、方剂1组、方剂2组。中药方剂(生理盐水)灌胃前后使用Morris水迷宫分别行定位航行和空间搜索试验。结果:随着时间延长,三组实验小鼠的潜伏期均呈缩短趋势,但是仅有方剂2组用药前后的潜伏期时长具有显著性差异,并比对照组、方剂1组明显缩短。用药后,方剂2组小鼠游泳总距离缩短、平均速度变慢、在站台所在象限停留时间延长、穿越站台所在位置次数明显增多,均具有统计学意义。结论:方剂2理气化痰降浊法经验方具有改善AD转基因小鼠学习记忆能力的作用。
OBJECTIVE: To compare the learning and memory abilities of APP / PS1 transgenic mice with Alzheimer’s disease (APP) by using two different prescriptions of Wenpipnshen (prescription 1) and Liqi phlegm turbid (prescription 2) prescriptions Study to explore the possibility of two Chinese medicine prescriptions for the treatment of AD. Methods: 24 AD mice were randomly divided into control group, prescription group 1 and prescription group 2. Chinese medicine prescription (saline) before and after gavage were used Morris water maze positioning navigation and space search test. Results: With the extension of time, the incubation period of three groups of mice showed a shortening trend, but only the prescription group 2 before and after treatment of the incubation period has significant difference, and compared with the control group, prescription group 1 was significantly shortened. After treatment, the total swimming distance between the two prescriptions group mice was shortened, the average speed was slower, the time spent in the quadrant of the platform was prolonged, and the frequency of passing through the platform was significantly increased, both of which were statistically significant. Conclusion: Prescription 2, Qi-tonifying, Phlegm-resolving and Turbidity method can improve the learning and memory ability of AD transgenic mice.