论文部分内容阅读
采用常规熔炼方法在氩气保护和电磁搅拌条件下制备了纳米羟基磷灰石(nHA)/Mg-2.5Zn-0.5Zr(质量分数,%)生物复合材料,并与基体镁合金对比,通过与大鼠成骨细胞共培养考察材料的体外生物相容性。结果表明,2组材料均无明显细胞毒性,但在倒置相差显微镜下,nHA/Mg-2.5Zn-0.5Zr材料周边细胞形态完好,密度显著高于基体镁合金组,前3天培养液中基本无材料溶出物,5~7天后溶出物少于基体镁合金组。细胞生长曲线显示,随着培养时间延长2组材料表面的细胞数量均呈上升趋势,而在复合培养的第3天和第5天,nHA/Mg-2.5Zn-0.5Zr复合材料表面细胞增殖量显著大于基体镁合金组。扫描电镜下可见成骨细胞对2组材料的反应有所不同,在复合材料表面,细胞呈梭形或三角形紧密贴伏,其伪足正常伸展使细胞彼此相连,7天后完全覆盖了整个试样。相反,基体镁合金表面细胞呈纺锤状,显示与材料的细胞吸附性较差。
The nano-hydroxyapatite (nHA) /Mg-2.5Zn-0.5Zr (mass fraction,%) bio-composite was prepared by conventional melting method under the conditions of argon protection and electromagnetic stirring. Compared with the matrix magnesium alloy, In Vitro Biocompatibility of Rat Osteoblasts Co-Cultured Materials. The results showed that there was no obvious cytotoxicity in the two groups of materials, but under inverted phase contrast microscope, the morphology of cells around the nHA / Mg-2.5Zn-0.5Zr material was intact and the density was significantly higher than that of the matrix magnesium alloy group. No material dissolution, 5 to 7 days after the dissolution of less than the matrix magnesium alloy group. The cell growth curve showed that the number of cells on the surface of the two groups of materials increased with the prolongation of culturing time. On the 3rd and 5th day of compound culture, the cell proliferation on the surface of nHA / Mg-2.5Zn-0.5Zr composite Significantly larger than the matrix magnesium alloy group. Scanning electron microscopy showed that the response of osteoblasts to the two materials was different. On the surface of the composite, the cells were spindle-shaped or triangular closely attached, and their pseudopods extended normally to connect the cells to each other. After 7 days, the whole sample was completely covered . In contrast, cells on the surface of the matrix magnesium alloy were spindle-shaped, showing poor cell adsorption with the material.