用EcoRI/XhoI两种限制性内切酶在同一反应体系中对TGFβ1 cDNA重组质粒联合酶切获得 1 3Kb的酶切片段作为裸探针 ,比以往文献中报道的长度缩短约 70 0bp,经地高辛标记获得较高的标记效率。本方法简化了操作步骤 ,并大大降低了cDNA探针的制备成本 ,不失为制备TGFβ1 cDNA探针的好方法 ,为TGFβ1 mRNA的检测提供了较好的工具。 The restriction endonuclease EcoRI / XhoI in the same reaction system TGFβ1 cDNA recombinant plasmid combined digestion to obtain a 13Kb digested fragment as a naked probe, compared with the length of the previous literature reported shortened about 70 0bp, The digoxigenin labeling achieves higher labeling efficiency. The method simplifies the operation procedure and greatly reduces the preparation cost of the cDNA probe, which is a good method for preparing the TGFβ1 cDNA probe and provides a good tool for the detection of the TGFβ1 mRNA.