目的探索应用重组E.coliTB1摇瓶生产日本血吸虫Sj28GST重组抗原的最适工艺条件。方法用2YT培养基进行发酵,观察培养温度、种子液接种量、诱导剂异丙基-β-D硫代半乳糖苷(IPTG)浓度、IPTG诱导时间等培养条件。结果培养温度为37℃,种子液接种量为10%,转速为200 r/m in,用0.8 mmol/L IPTG在开始发酵4 h后进行诱导,为最佳发酵条件。在此条件下,振荡培养17 h后,细菌密度达到吸光度(A600)值2.5,重组日本血吸虫Sj28GST产量达到21.6mg/L发酵液。结论成功探索了日本血吸虫Sj28GST重组抗原的摇瓶发酵条件。 Objective To explore the optimum conditions for producing recombinant antigen of Sj28GST from Schistosoma japonicum by using recombinant E. coli TB1 shake flask. Methods Fermentation was carried out in 2YT medium. The culture conditions such as incubation temperature, inoculum size, IPTG concentration and IPTG induction time were observed. Results The optimum fermentation conditions were as follows: culture temperature 37 ℃, inoculum size 10%, rotation speed 200 r / min, induction with 0.8 mmol / L IPTG for 4 h. Under these conditions, the bacterial density reached the absorbency (A600) value of 2.5 after 17 h of shaking cultivation, and the yield of recombinant Sj28GST reached 21.6 mg / L. Conclusion The shake flask fermentation conditions of Sj28GST recombinant antigen of S. japonicum were successfully explored.