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目的 :观察外周血淋巴细胞rDNA转录活性在人体疾病过程中的动态变化 ,探讨其对肿瘤诊断和治疗监测的意义。方法 :用图像分析系统分析 10 4例癌瘤、心肌梗死、心肌炎患者的外周血淋巴细胞AgNor银染强度 (用以表示rDNA转录活性 )。结果 :癌瘤、心肌梗死、心肌炎患者rDNA转录活性明显降低。癌瘤患者手术后比手术前rDNA转录活性明显降低 ,rDNA转录活性与癌组织DNA >5倍体值呈负相关。结论 :淋巴细胞rDNA转录活性是人体免疫反应的灵敏标志 ,具有广泛的临床应用价值。可作为肿瘤诊断和治疗监测的参考指标 ,但缺乏特异性。
Objective : To observe the dynamic changes of rDNA transcriptional activity of peripheral blood lymphocytes in human diseases and to explore its significance for tumor diagnosis and therapeutic monitoring. METHODS: The silver staining intensity of AgNor in lymphocytes of peripheral blood of 104 patients with carcinoma, myocardial infarction, and myocarditis was analyzed by an image analysis system (used to indicate rDNA transcription activity). RESULTS: The rDNA transcription activity was significantly reduced in patients with carcinoma, myocardial infarction, and myocarditis. In patients with cancer, the transcriptional activity of rDNA was significantly lower than before surgery. The transcriptional activity of rDNA was negatively correlated with the DNA >5-fold value of cancer tissue. Conclusion: The transcriptional activity of lymphocyte rDNA is a sensitive marker of human immune response and has extensive clinical application value. Can be used as a reference indicator for tumor diagnosis and treatment monitoring, but lacks specificity.