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目的 探讨白细胞介素 12抑制裸鼠肝癌中血管生成素 2 (Ang 2 )和血管内皮生长因子(VEGF)基因表达及其意义。方法 将小鼠白细胞介素 12基因转染小鼠肝癌H 2 2细胞后,接种到裸鼠肾包膜下,第11天取出肿瘤组织。通过逆转录 聚合酶链反应(RT PCR)观察实验组和对照组瘤组织中Ang 2和VEGF基因在mRNA水平上的差异。结果 实验组Ang 2和VEGF基因的PCR产物条带亮度均弱于对照组(P <0 .0 5 ) ,实验组Ang 2 (0 .7878±0 .14 71)和VEGF(0 .8867±0 .192 4)基因的PCR产物条带辉度比值低于载体对照组(1.0 3 19±0 .15 74、1.1744±0 .1189)和空白对照组(1.0 3 61±0 .15 3 6、1.1874±0 .175 2 ,P <0 .0 5 )。结论 IL 12抑制Ang 2和VEGF基因在裸鼠肝癌组织中的表达,从而抑制了裸鼠肝癌的血管生成
Objective To investigate the effects of interleukin-12 on angiopoietin 2 (Ang 2) and vascular endothelial growth factor (VEGF) gene expression in human hepatocellular carcinoma (HCC) and their significance. Methods Mouse interleukin 12 gene was transfected into H 2 2 hepatoma cells of mice and inoculated into the renal capsule of nude mice. Tumor tissues were removed on the 11th day. The mRNA levels of Ang 2 and VEGF in the experimental group and the control group were observed by reverse transcriptase-polymerase chain reaction (RT PCR). Results The bands of PCR products of Ang 2 and VEGF gene in experiment group were weaker than those in control group (P <0.05). The levels of Ang 2 (0.7878 ± 0.14 71) and VEGF (0.8867 ± 0 .192 4) The PCR products of the gene band brightness ratio was lower than the carrier control group (1.0319 ± 0.1574,1.1744 ± 0.1189) and the blank control group (1.03 61 ± 0.153 6,1.1874 ± 0 .175 2, P <0. 05). Conclusion IL-12 inhibits the expression of Ang 2 and VEGF in human hepatocellular carcinoma in nude mice and thus inhibits angiogenesis