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目的:探讨递增运动负荷训练后大鼠心室肌蛋白质组的表达特征,为进一步筛选对运动性疲劳的研究具有重要意义的蛋白提供实验依据。方法:将10只SD雄性大鼠随机分为对照组和实验组。实验组经过7周的递增运动负荷训练(最后一次为力竭)后与对照组同时麻醉处死,取心室肌,提取心肌组织的全蛋白进行双向凝胶电泳分离。运用Bio-radPDquest图像分析软件对图谱进行分析。结果与结论:在电泳图谱上运动组可见蛋白质点338±17个,对照组可见352±17个。运动后差异表达的蛋白质点共有99个。运动后消失的点有5个,表达量上调4倍以上和下调幅度超过80%的点各12个,表达量上调1倍以上和下调幅度超过50%的点分别有52个和42个。递增负荷运动训练后,大鼠心室肌蛋白质组发生了明显的质变和量变。运动后表达量差异比较大的蛋白质点的分子量主要集中在35～57kD,等电点主要集中在6.0～7.4和8.6～9.6,在这个范围的蛋白质多为能量代谢酶,提示心室肌能量代谢的改变可能是运动引起心肌疲劳的原因。
OBJECTIVE: To investigate the expression characteristics of rat ventricular muscle proteome after exercise training with increasing exercise load to provide experimental evidence for further screening of proteins of importance for the study of exercise-induced fatigue. Methods: Ten SD male rats were randomly divided into control group and experimental group. The experimental group was subjected to progressive exercise load training (last exhaustive) for 7 weeks and then anesthetized and sacrificed at the same time with the control group. The ventricular muscle was taken out and the whole protein in the myocardium was extracted for two-dimensional gel electrophoresis separation. Bio-radPDquest image analysis software to analyze the map. RESULTS AND CONCLUSION: In the electrophoretogram, there were 338 ± 17 protein spots in the exercise group and 352 ± 17 in the control group. A total of 99 protein spots differentially expressed after exercise. There were 5 spots that disappeared after exercise, 12 spots whose expression levels were up 4 times and down more than 80% respectively, 52 points and 42 spots respectively whose expression levels were up 1 times and down more than 50%. Incremental load exercise training, rat ventricular muscle proteome obvious qualitative changes and quantitative changes. After the exercise, the molecular weight of protein spots with large difference in expression mainly concentrated in 35-57kD, and the isoelectric points mainly concentrated in 6.0-7.4 and 8.6-9.6. Proteins in this range were mostly energy metabolism enzymes, suggesting that the energy metabolism Changes may be the cause of exercise-induced myocardial fatigue.