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本文报告711条美洲钩虫成虫在幼犬血清、199培养基、小牛血清、台氏液等七种培养基中的培养结果。其中以幼犬血清和含少量199培养基的幼犬血清培养效果为好,虫体在体外存活半月以上者占64.2％,一月以上者占21％,雌虫最长存活52天,雄虫43天,并有虫体交配,在培养基中查见了受精卵、未受精卵和钩虫幼虫。本实验体外培养美洲钩虫成虫成功,为研究美洲钩虫各个发育阶段创造了一定条件,为探索人体两种钩虫的差异提供了一条途径。
This article reports the results of 711 adult hookworm adults cultured in puppies, 199 medium, calf serum, Tyrode’s medium. Among them, the serum of puppies and puppies containing a small amount of 199 medium had the best culture effect, while the worms survived more than half a month in vitro, accounting for 64.2%, 21% more than one month, and the longest females surviving for 52 days. 43 days, mating with parasites, fertilized eggs, unfertilized eggs and hookworm larvae were observed in the medium. The success of this experiment in vitro culture of hookworm adults, for the development of hookworm in various stages of development to create certain conditions, in order to explore the difference between the two kinds of human hookworm provides a way.