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不同生长季节的黄连外植体在一系列培养基上诱导产生愈伤组织．经高效液相色谱法测定愈伤组织内小檗碱和药根碱的含量．结果表明，幼叶柄外植体在６７－Ｖ０．５ｍｇ／Ｌ２，４－Ｄ上产生的愈伤组织的小檗碱和药根碱含量明显高于对照组．花苔外植体在６７－Ｖ０．５ｍｇ／Ｌ２，４－Ｄ上诱导的愈伤组织小檗碱含量高于对照组．
Coptis explants from different growing seasons induce callus production on a range of media. Determination of berberine and jatrorrhizobium in callus by high performance liquid chromatography. The results showed that the contents of berberine and jatrorrhizine in callus induced by young petiole explants on 67-V0.5mg / L 2,4-D were significantly higher than those in control group. The content of berberine in calli induced by 67-V0.5mg / L 2,4-D was higher than that of the control group.