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本文检测并描述了一种以前未被认识的同时具有髓系和自然杀伤细胞特征的急性白血病亚型。作者从350例成人AML中测得20例(占6％),它们均具有一致的免疫表型CD33~+、CD56~+、CD11a~+、CD13(低表达)、CD15(低表达)、CD34~±、HLA-DR~-、CD16~-。应用多色流式细胞计数仪分析,结果更有力地证明了髓系分化抗原(CD33、CD13、CD15)和NK细胞相关抗原(CD56)的同时表达。反转录PCR(Rt-PCR)分析也充分地显示出CD56在白血病幼稚细胞中的一致性。除2例表达CD4以外,没有1例表达CD2、CD3或CD8,并且未检出TCR基因重排。多数病例的白血病细胞均有特殊的形态学特征(核膜深陷,胞浆量少并伴有纤细的嗜苯胺蓝颗粒和细的苏丹黑B颗粒以及髓性过氧化物酶阳
A previously unknown acute leukemia subtype with both myeloid and natural killer cell characteristics was tested and described herein. The authors measured 20 (accounting for 6%) of 350 adult AML patients. They all had consistent immunophenotypes of CD33+, CD56+, CD11a+, CD13 (low expression), CD15 (low expression), and CD34. ~±, HLA-DR~-, CD16~-. Using multicolor flow cytometric analysis, the results demonstrated more strongly the simultaneous expression of myeloid differentiation antigens (CD33, CD13, CD15) and NK cell-associated antigens (CD56). Reverse transcription PCR (Rt-PCR) analysis also fully demonstrated the consistency of CD56 in leukemic naive cells. Except for 2 cases of CD4 expression, none of them expressed CD2, CD3, or CD8, and no TCR gene rearrangement was detected. In most cases, leukemic cells have special morphological features (nuclear membrane subsidence, small cytoplasm with fine amphetamine-blue particles and fine Sudan Black B particles and myeloperoxidase