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采用亲和毛细管电泳的配体分离模式,以激光诱导荧光作为检测手段,测定了荧光素标记的孕酮与孕酮单克隆抗体之间的结合常数,并研究了温育时间、电泳条件等因素对测定的影响。
Affinity capillary electrophoresis with ligand separation mode, laser-induced fluorescence as a detection means, the determination of the binding constant of fluorescein-labeled progesterone and progesterone monoclonal antibody, and the incubation time, electrophoresis conditions and other factors Impact on the assay.