对测定低浓度猪胰弹性酶的四种不同方法精确度的评价,其中之一是用一种合成底物,其余三种方法是用天然底物弹性蛋白。最精确的方法采用的合成底物Succ(Ala)_3NA(即:N-琥珀酰-L-三丙氯酸-对硝基苯胺。译者注)(批内变异系数=1.9％,批间变异系数=2.5％);利用弹性蛋白为底物的方法中最精确的是荧光素标记弹性蛋自(批内变异系数=5.1％,批间变异系数=5.5％)。 One of the four methods used to determine the accuracy of porcine pancreatic elastase at low concentrations was one synthetic substrate and the other three were native substrate elastin. The most accurate method uses the synthetic substrate Succ (Ala) _3NA (intra-assay coefficient of variation = 1.9%, inter-assay variation Coefficient = 2.5%); the most accurate method using elastin as a substrate was fluorescein-labeled elastin (intra-assay CV = 5.1%, inter-assay CV = 5.5%).