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目的研究难治性癫患者脑组织内突触囊泡循环再生相关蛋白的表达,以探讨难治性癫的成因及其他可能的发病机制。方法用免疫组化、Western印迹法检测与突触囊泡循环再生密切相关的网格蛋白(clathrin)、突触囊泡膜蛋白Ⅰ(synaptotagminⅠ)在难治性癫患者脑部的表达,并与健康人群比较。结果免疫组化显示与突触囊泡循环再生密切相关的网格蛋白[在海马为(0·173±0·019),颞叶为(0·186±0·024)]、突触囊泡膜蛋白Ⅰ[在海马为(0·188±0·019),颞叶为(0·190±0·017)]在难治性癫患者脑部表达较健康人增强(P<0·05),Western印迹显示网格蛋白、突触囊泡膜蛋白Ⅰ在难治性癫组增加,电泳条带明显增宽,且免疫组化显示难治性癫患者脑组织中网格蛋白、突触囊泡膜蛋白Ⅰ表达随病程的延长而有增强的趋势。结论难治性癫患者脑组织内存在突触囊泡循环再生过程强化。
Objective To study the expression of synaptic vesicle cycle-related protein in brain tissue of patients with refractory epilepsy and to explore the possible causes and other possible pathogenesis of refractory epilepsy. Methods Immunohistochemistry and Western blotting were used to detect the expression of clathrin, synaptotagmin Ⅰ in the brain of patients with refractory epilepsy Compared with healthy people. Results Immunohistochemistry showed that the number of clathrin ([(0.173 ± 0.190) in the hippocampus and (0.186 ± 0.244) in the temporal lobe) closely related to synaptic vesicle cycle regeneration, synaptic vesicles Membrane protein Ⅰ (0. 188 ± 0. 019 in the hippocampus and (0.190 ± 0.017) in the temporal lobe) was more expressed in the brains of patients with refractory epilepsy than in healthy subjects (P <0.05) ), Western blotting showed that the clathrin, synapsin I protein increased in the refractory epilepsy group, the electrophoresis band was significantly broadened, and the expression of clathrin, Synaptic vesicle protein Ⅰ expression with the extension of the course there is an increasing trend. Conclusion The process of synaptic vesicle recycling in brain tissue of patients with refractory epilepsy is intensified.