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目的研究氟接触工人外周血淋巴细胞的遗传毒性。方法将广西某铝厂160名氟接触男性工人分为净化车间组(80名)和电解铝车间组(80名),另选该厂非氟接触男性工人60名为对照组。用氟离子选择电极法测定生产车间中氟化物的浓度及各组工人血清中氟浓度,单细胞凝胶电泳法(SCGE)检测工人淋巴细胞中DNA损伤程度,用流式细胞术(FCM)检测细胞凋亡率。结果与对照组相比,净化车间组和电解铝车间组工人血清中氟浓度、淋巴细胞彗星发生率、彗尾DNA百分率、彗星尾长、细胞凋亡率分别增加了88.9%、139.6%、230.8%、135.6%、128.4%和60.0%、72.5%、85.2%、88.3%、100.9%,差异均有统计学意义(P<0.05)。将净化车间组80名氟接触工人按接氟工龄分为3个组(即<10、10~15和>15a工龄组)。校正年龄,吸烟水平后,与氟作业低工龄组比较,中、高工龄组的彗星细胞率、彗尾DNA百分率、彗星尾长分别增加了50.5%、112.5%、47.0%和74.4%、127.8%、65.6%,组间比较,差异有统计学意义(P<0.01或P<0.05)。结论职业性氟接触可导致作业工人外周血淋巴细胞中DNA损伤,诱发淋巴细胞凋亡增加,提示氟具有明显的遗传毒性。
Objective To study the genotoxicity of peripheral blood lymphocytes in workers exposed to fluoride. Methods A total of 160 fluoride exposed male workers from an aluminum factory in Guangxi were divided into two groups: 80 for purification workshop and 80 for electrolytic aluminum workshop, and 60 for non-fluoride male workers. Fluoride ion-selective electrode method was used to determine the concentration of fluoride in the production workshop and the concentration of fluoride in the serum of each group of workers. The degree of DNA damage in the workers’ lymphocytes was detected by single cell gel electrophoresis (SCGE). Flow cytometry (FCM) Rate of apoptosis. Results Compared with the control group, the levels of fluoride, the incidence of lymphocyte comet, the percentage of tail DNA, the tail of tail, and the apoptosis rate in serum of workers in purification workshop and electrolytic aluminum workshop increased by 88.9%, 139.6% and 230.8 %, 135.6%, 128.4% and 60.0%, 72.5%, 85.2%, 88.3% and 100.9%, respectively. The difference was statistically significant (P <0.05). Eighty fluorine-exposed workers in the purification plant group were divided into three groups according to the fluoride occupation (ie, <10, 10-15 and> 15a). After adjusting for age and smoking level, the percentages of comet cells, comet DNA, and tail of comet were increased by 50.5%, 112.5%, 47.0%, 74.4% and 127.8%, respectively, , 65.6%. There was significant difference between the two groups (P <0.01 or P <0.05). Conclusion Occupational fluoride exposure may lead to DNA damage in peripheral blood lymphocytes of workers and increase the apoptosis of lymphocytes, suggesting that fluorine has obvious genotoxicity.