论文部分内容阅读
从列文虎克到21世纪,显微镜由一个看似牢不可破的原则所控制:分辨两个对象的能力受限于观察它们的光波波长。但在2000年,研究人员显示出,这种所谓的衍射极限可以被打破,在接下来的十年中揭示了从GSDIM和PALM到SIM、STED和STORM的一系列像“字母汤”一样的超分辨率技术。不,这并不是审稿编辑出了问题,这句话末尾真的有两个句号。。你的大脑抓住了这个明显的错误,因为你眼睛晶状体所具备的分辨能力足以区分两个紧挨着的物体。当然,显微镜可以解析更细微的细节,但这种能力不是无限的。所谓的阿贝(或衍射)极限限制着显微镜的能力,而且在某种情况下,两个物体会显示为一个。
From Leavenon to the 21st century, the microscope is dominated by a seemingly unbreakable principle: the ability to distinguish between two objects is limited by the wavelength of their light waves. But in 2000, researchers showed that this so-called diffraction limit could be broken, revealing in a series of lines like GSDIM and PALM to SIM, STED and STORM over the next decade Super-resolution technology. No, this is not a problem with manuscript editors. There are really two periods at the end of this sentence. . Your brain captures this obvious mistake, because the resolution of your lens of the eye is sufficient to distinguish the two next objects. Of course, the microscope can resolve finer details, but this ability is not unlimited. The so-called Abbe’s (or diffraction) limits limit the ability of the microscope, and in some cases two objects will show up as one.