T4-RNA连接酶自1972年由Silber等人发现以来,已成为合成核酸的一种重要工具。我们还未见有关固相T4-RNA连接酶的报道。我们将T4-RNA连接酶偶联到Sepharose 4B的衍生物上,制备了一种固相T4-RNA连接酶。每毫克衍生物结合0.3单位连接酶(不是最大偶联量)。此固相酶可以催化ATP和~(32)PP_i间的交换反应。在反应液含20mM Tris-HCI(pH8.4),10mMDTT,20mMMgCl_2, T4-RNA ligase has been an important tool for the synthesis of nucleic acids since it was discovered by Silber et al in 1972. We have not seen any reports of solid-phase T4-RNA ligase. We coupled a T4-RNA ligase to a derivative of Sepharose 4B to prepare a solid-phase T4-RNA ligase. 0.3 units of ligase per milligram of derivative bound (not the maximum amount of conjugate). This enzyme can catalyze the exchange between ATP and ~ (32) PP_i. After the reaction solution contained 20 mM Tris-HCI (pH 8.4), 10 mM DTT, 20 mM MgCl 2,