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目的探求冻存复苏人卵巢皮质在裸鼠体内的发育及卵细胞的提取。方法取手术切除的5例患者的卵巢皮质,放入1.5mol/L的二甲基亚砜+10%血清中,应用可控程序冻冰仪逐渐降温,移入液氮中冻存。3个月后,将融解的卵巢皮质移植入90只裸鼠皮下,注射hFSH12周,刺激卵泡生长。注射hCG36h后,用18号针头穿刺提取卵细胞。结果卵巢直径为3～9mm,穿刺获19个卵细胞,放入TC-199培养基,加20%胎牛血清,25mmol/L丙酮酸,75mIU/mLFSH和LH培养,获15个分裂中期的次级卵母细胞。结论人卵巢组织冻融后可异体移植生长,且其卵细胞可在体外发育成熟。
Objective To explore the development of cryopreserved human ovarian cortex in nude mice and the extraction of egg cells. Methods Five cases of ovariectomized patients were placed in 1.5mol / L dimethylsulfoxide + 10% serum and gradually cooled with liquid nitrogen. Three months later, the melted ovary cortex was subcutaneously transplanted into 90 nude mice and injected with hFSH for 12 weeks to stimulate follicle growth. After injection of hCG for 36 h, the 18-gauge needle was used to extract the egg cells. Results The diameter of ovary was 3 ~ 9mm, and 19 eggs were punctured. The ovaries were cultured in TC-199 medium supplemented with 20% fetal bovine serum, 25mmol / L pyruvate, 75mIU / mL FSH and LH. Oocyte. Conclusion Human ovarian tissue can be allotransplantated after freezing and thawing, and its egg cells can mature in vitro.