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目的建立测定SD大鼠体内雷帕霉素(RAPA)血药浓度的HPLC-MS/MS联用方法。方法色谱柱为Phenomenex LunaC18(2.0 mm×250 mm,5μm);以甲醇-水(90∶10)为流动相;流速0.25 mL.min-1;柱温55℃。选用ESI源,以多反应采集模式(MRM)进行正离子检测,RAPAm/z936.6→409.2和丙酸睾酮(内标)m/z345.4→109.1。取全血样品经液-液萃取后,HPLC进样10μL。结果RAPA的线性范围为0.54~108.00μg.L-1,定量下限为0.54μg.L-1,日内、日间精密度(RSD)均小于12%。结论本方法精密、准确,适用于RAPA在大鼠体内的药动学研究。
Objective To establish a HPLC-MS / MS method for the determination of plasma concentration of rapamycin (RAPA) in SD rats. Methods The chromatographic column was Phenomenex Luna C18 (2.0 mm × 250 mm, 5 μm). The mobile phase was methanol-water (90:10). The flow rate was 0.25 mL.min-1. The column temperature was 55 ℃. Positive ion detection was performed with multiple reaction collection mode (MRM) using ESI source, RAPAm / z936.6 → 409.2 and testosterone propionate (internal standard) m / z 345.4 → 109.1. After whole blood samples were taken by liquid-liquid extraction, 10 μL was injected by HPLC. Results The linear range of RAPA was 0.54 ~ 108.00μg.L-1, and the lower limit of quantitation was 0.54μg.L-1. The intra-and inter-day precision was less than 12%. Conclusion This method is accurate and accurate and is suitable for the pharmacokinetic study of RAPA in rats.