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为了从神经营养因子角度探讨移植修复机理，将大鼠１４ｄ胚胎脊髓（ＥＳＣ）植入急性损伤成鼠脊髓后１、３、５、７、１０、１５和３０ｄ，用原位杂交和斑点杂交技术对ＥＳＣ和宿主脊髓（ＨＳＣ）内ＮＴ－３ｍＲＮＡ的变化进行定性和定量观察。定性观察显示，在正常脊髓内，ＮＴ－３ｍＲＮＡ以前角运动神经元和少量胶质细胞分布为主；脊髓损伤以后，杂交产物扩大到中小型神经元，同时更多的胶质细胞参与了反应；ＥＳＣ植入后，除移植物本身继续表达外，宿主脊髓阳性反应神经元和胶质细胞数量进一步增加。定量结果表明，损伤组原位杂交和斑点杂交的反应强度明显高于正常组；而移植组的分子杂交反应又在很多时相点上显著高于损伤组。除此而外，分子杂交反应在损伤组和移植组内持续的时间也不相同，前者的最强反应期为术后７ｄ，后者为术后１０～１５ｄ．我们认为，移植的ＥＳＣ除为自身和ＨＳＣ提供神经营养外，也诱发了ＨＳＣ在发生过程中曾经拥有的合成机制，以增强ＮＦ表达的方式为自身再生提供营养，同时也为移植物的发育分化提供合适的营养环境。
In order to explore the mechanism of transplantation repair from the point of neurotrophic factor, embryonic spinal cord (ESC) was implanted into rat spinal cord at 1, 3, 5, 7, 10, 15 and 30 days after acute injury to rat spinal cord by in situ hybridization and dot blot hybridization Qualitative and quantitative observations of NT-3 mRNA changes in ESC and host spinal cord (HSC) were performed. Qualitative observation showed that in the normal spinal cord, NT-3 mRNA mainly distributed in anterior horn motoneurons and few glial cells; after spinal cord injury, the hybridization products expanded to small and medium sized neurons and more glial cells were involved in the reaction; After ESC implantation, the number of spinal cord-positive neurons and glial cells in the host increased further, except for the graft itself. The quantification results showed that the reaction intensity of in situ hybridization and dot blot hybridization in the injury group was significantly higher than that in the normal group; however, the molecular hybridization reaction in the transplantation group was significantly higher than that in the injury group at many time points. In addition, the molecular hybridization reaction in the injury group and the transplantation group continued for a different time, the strongest response time for the former 7d after surgery, the latter for 10 ~ 15d. In our opinion, transplanted ESC, in addition to providing neurotrophic for itself and HSC, also induces a synthetic mechanism that once existed in the course of HSC and enhances the expression of NF in order to provide nutrition for self-regeneration and also for the developmental differentiation of the graft Provide a suitable nutrition environment.