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用120个随机引物对4种常见根结线虫10个小种和类型进行了全基因组随机扩增DNA多态性（RAPD）分析，筛选出的11个适宜引物共扩增出91条RAPD谱带，86条是多态性谱带，占总谱带的94．5％；OPL12、OPK01对4种根结线虫种及其小种扩增的谱型有明显的特异性。聚类分析显示在种间水平上4种根结线虫中花生根结线虫和爪哇根结线虫亲缘关系最近，遗传距离为0．532，北方根结线虫与另外3种根结线虫的亲缘关系最远，平均遗传距离为0．786；种下水平上同种根结线虫的不同小种和类型间存在不同程度的遗传差异，南方根结线虫4个生理小种间，花生根结线虫2个生理小种间亲缘关系较近，爪哇根结线虫2个酯酶谱带类型间，北方根结线虫2个细胞生物学小种间遗传差异较大。在RAPD技术的基础上探索根结线虫分类鉴定的分子方法有着良好的前景。
Random amplified polymorphic DNA (RAPD) analysis was performed on 10 races and 4 genotypes of four common root knot nematodes using 120 random primers. A total of 91 RAPD bands were amplified by 11 suitable primers , 86 were polymorphic bands, accounting for 94.5% of the total bands. OPL12 and OPK01 had obvious specificity to four genotypes of root-knot nematode and their races. Cluster analysis showed that among the four root-knot nematodes, the root-knot nematode and the root-knot nematode were most closely related at the interspecific level, with a genetic distance of 0.532. The genetic relationship between the root-knot nematode and the other three root knot nematodes And the average genetic distance was 0.786. There were some genetic differences among different races and types of the same root-knot nematode at the species level. There were four physiological races of Meloidogyne incognita, two root-knot nematode peanut Physiological races were closely related to each other. Two Eschromostromes strains of the genus Meloidogyne javanica and two genomic variants of the genus Meloidogyne spp. On the basis of RAPD technology, it has a good prospect to explore molecular methods of identification and identification of root-knot nematode.