论文部分内容阅读
目的:探讨IgA肾病(IgA nephropathy,IgAN)患者血清中血小板源性生长因子BB(PDGF-BB)与肾脏不同病理改变的关系,旨在寻找一种能反映IgAN肾损害程度的非创伤性临床检测指标。方法:应用酶联免疫吸附法(ELISA)测定92例IgAN患者、30例微小病变性肾病(MCD)患者、30例健康对照的PDGF-BB水平并进行比较;将92例IgAN患者的肾脏病理按Lee氏分级分为A组(Lee氏I级或II级)16例,B组(Lee氏III级)43例,C组(Lee氏IV级或V级)33例,并进行katafuchi半定量评分,观察血清PDGF-BB水平与IgAN不同肾脏病理改变的关系。结果:(1)IgAN患者血清PDGF-BB的含量(746.530±293.012)ng/L明显高于MCD组(208.910±118.56)ng/L及正常对照组(215.320±124.064)ng/L(P<0.05);MCD组(208.910±118.56)ng/L与正常对照组(215.320±124.064)ng/L对比无统计学意义(P>0.05);(2)IgAN者中血清PDGF-BB的浓度C组(926.443±237.162)ng/L明显高于B组(693.103±284.608)ng/L及A组(519.043±198.671)ng/L(P<0.05);同时B组(693.103±284.608)ng/L高于A组(519.043±198.671)ng/L(P<0.05);(3)Pearson相关分析:血清PDGF-BB水平与平均动脉压、血肌酐、24h尿蛋白定量,尿素氮均呈正相关(r=0.353,0.342,0.270,0.261;P均<0.05);与肾小球滤过率呈负相关性(r=-0.414,P<0.05);(4)多元线性回归分析:平均动脉压及Lee氏分级是血清PDGF-BB升高的独立危险因素。结论:血清PDGF-BB浓度可反映IgAN肾脏病理改变的严重程度,可做为IgAN肾损害的非创伤性临床检测指标。
Objective: To investigate the relationship between platelet-derived growth factor BB (PDGF-BB) and pathological changes of kidney in IgA nephropathy (IgAN) patients and to find a noninvasive clinical test that can reflect the degree of IgAN nephropathy index. Methods: The levels of PDGF-BB in 92 patients with IgAN, 30 patients with minimal change nephropathy (MCD) and 30 healthy controls were determined by enzyme-linked immunosorbent assay (ELISA). The pathological changes of 92 patients with IgAN Lee’s classification was divided into group A (Lee’s grade I or II) in 16 cases, group B (Lee’s grade III) in 43 cases, group C (Lee’s grade IV or V) in 33 cases, and katafuchi semi-quantitative score , Observe the relationship between serum PDGF-BB levels and IgAN pathological changes in different kidneys. Results: (1) The serum level of PDGF-BB in IgAN patients (746.530 ± 293.012) ng / L was significantly higher than that in MCD group (208.910 ± 118.56) ng / L and the normal control group (215.320 ± 124.064) ng / L (208.910 ± 118.56) ng / L and 215.320 ± 124.064 ng / L in MCD group (P> 0.05). (2) Serum concentration of PDGF-BB in IgAN group C (693.103 ± 284.608) ng / L and group A (519.043 ± 198.671) ng / L respectively (P <0.05); while group B (693.103 ± 284.608) ng / L was higher than that of group B (519.043 ± 198.671) ng / L (P <0.05). (3) Pearson correlation analysis: Serum PDGF-BB level was positively correlated with mean arterial pressure, serum creatinine, 24h urinary protein and urea nitrogen (r = 0.353 , 0.342,0.270,0.261; P <0.05), and glomerular filtration rate (r = -0.414, P <0.05). (4) Multiple linear regression analysis: mean arterial pressure and Lee’s grading Is an independent risk factor for elevated serum PDGF-BB. Conclusion: Serum PDGF-BB concentration can reflect the severity of IgAN renal pathological changes, can be used as noninvasive clinical indicators IgAN renal damage.