对近年来同期住院的70例十二指肠球部溃疡患者进行了血浆胃动素的测定,现将结果报告如下。 1 对象和方法 1.1 对象 男60例,女10例,年龄17—71岁,平均年龄33.3岁。均排除急性感染、肝病、糖尿病,应用激素或避孕药等其它药物。全部患者均内镜检查,证实为十二指肠球部溃疡活动期。另外选择31例健康男性作为对照组,年龄在20—40岁,平均年龄32.6岁。 1.2 方法 内镜证实为十二指肠球部溃疡活动期的70例患者。在内镜检查次日抽血做胃动素测定,其中40例患者在内镜检查结束后即抽血做上述激素的测定。全部患者在镜检前及抽血前未用解痉药及止痛剂。另外有20例患者在内镜检查证实十二指肠球部溃疡愈合后,1—3d内进行了胃动素的测定。用解放军总医院基础所兴华生物新技术开发中心所提供的胃动素放射免疫分析试剂盒,清晨空腹或镜检后自肘静脉采血2ml,置于30ml 10％EDTA二钠和30ml抑肽酶玻璃管中混匀,4℃离心,取血浆-20℃以下保存。为避免血浆中冷不凝蛋白对测定的干扰,测定时在血浆复溶 In 70 cases of hospitalized in recent years, duodenal ulcer in patients with plasma motilin determination, the results reported below. 1 objects and methods 1.1 object male 60 cases, 10 females, aged 17-71 years, with an average age of 33.3 years. All except acute infection, liver disease, diabetes, hormones or contraceptives and other drugs. All patients were endoscopically confirmed duodenal ulcer activity. Another 31 healthy men as control group, aged 20-40 years old, with an average age of 32.6 years old. 1.2 Methods Endoscopic confirmed duodenal ulcer activity in 70 patients. On the second day of endoscopy, blood was taken for determination of motilin. Forty patients underwent a blood test to determine the above hormones after endoscopy. All patients without microspheres and analgesics before microscopic examination and before blood sampling. Another 20 patients in the endoscopic examination confirmed duodenal ulcer healing, 1-3d motilin determination. Using the motilin radioimmunoassay kit provided by Xinghua New Bio-technology Development Center of PLA General Hospital, 2ml blood samples were taken from the cubital vein after fasting or microscopic examination in 30ml of 10% disodium EDTA and 30ml aprotinin Mix well in a glass tube and centrifuge at 4 ° C for preservation below -20 ° C. In order to avoid the interference of plasma non-condensing protein in the determination of plasma coagulation in the assay