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观察新型光敏剂5-氨基乙酰丙酸(ALA)介导的光动力学疗法(PDT)治疗Fischer 344大鼠9L胶质瘤的效果并探讨其作用机制。20只雄性Fischer 344大鼠颅内种植9L脑胶质瘤细胞后,随机分为对照组、PDT治疗组(40J/cm2组、80J/cm2组和120J/cm2组)。接种9L细胞7天后治疗组接受相应的ALA-PDT治疗。治疗后第7天处死所有动物,通过Hematoxylin-Eosin(H-E)染色法检测颅内肿瘤的生长情况;利用免疫组化法检测肿瘤细胞的增殖活性(Ki67)和TUNEL法测定肿瘤细胞的凋亡。结果表明利用不同能量的ALA-PDT治疗后大鼠的生存状态明显改善,脑胶质瘤的体积减小,其治疗效果在一定范围内随着能量的增大而增强,但80J/cm2和120J/cm2的治疗效果基本相同。通过对肿瘤细胞增殖和凋亡的检测发现,ALA-PDT对细胞的增殖无影响,但可诱导肿瘤细胞的凋亡。ALA-PDT通过诱导肿瘤细胞的凋亡可有效治疗脑胶质瘤。
To observe the effect of novel photosensitizer 5-aminolevulinic acid (ALA) -mediated photodynamic therapy (PDT) on 9L glioma in Fischer 344 rats and to explore its mechanism. Twenty male Fischer 344 rats were intracranially inoculated with 9L glioma cells and then randomly divided into control group, PDT treatment group (40J / cm2 group, 80J / cm2 group and 120J / cm2 group). After 7 days of inoculation of 9L cells, the treatment group received the corresponding ALA-PDT treatment. All animals were sacrificed on the 7th day after treatment, and the growth of intracranial tumors was detected by Hematoxylin-Eosin (H-E) staining. The proliferation of tumor cells was detected by immunohistochemistry (Ki67) and apoptosis by TUNEL. The results show that the use of different energy ALA-PDT treatment significantly improved the survival of rats, brain glioma volume decreased, the therapeutic effect within a certain range with the increase of energy increased, but 80J / cm2 and 120J / cm2 treatment is basically the same effect. Through the detection of tumor cell proliferation and apoptosis, ALA-PDT showed no effect on cell proliferation, but could induce tumor cell apoptosis. ALA-PDT can effectively treat glioma by inducing tumor cell apoptosis.