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采用放射配体(125IBE2254)结合分析,证明狗胸主动脉平滑肌存在α1肾上腺素受体,其最大结合容量为199±31fmol/mg·蛋白,KD为139±27pmol/L,其中3/4以上属α1B亚型,另外1/4左右属α1A亚型。
Radioligand (125IBE2254) binding analysis showed that α1 adrenergic receptor was present in the smooth muscle of dog thoracic aorta. Its maximum binding capacity was 199 ± 31 fmol / mg · protein, KD was 139 ± 27 pmol / L, of which more than 3/4 α1B subtype, the other 1/4 about α1A subtype.