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目的:研究小儿防哮敷贴粉穴位敷贴对哮喘豚鼠血清IFN-γ和LXA4的影响。方法:将60只健康豚鼠随机分为正常组、模型组、治疗组1组和2组,每组15只。正常组自然喂养,其余三组采用卵蛋白致敏法建立哮喘豚鼠模型。治疗1组于哮喘造模第1天起,给予小儿防哮敷贴粉敷贴治疗,每日1次,每次30min,共10天;治疗2组哮喘造模,并于雾化激发第11天起,给予小儿防哮敷贴粉敷贴治疗,方法同治疗1组。实验结束后采用ELISA法测定血清IFN-γ和LXA4含量。结果:治疗1组、2组哮喘豚鼠引喘潜伏期较模型组均明显延长,有显著性差异(P<0.01);治疗1组、2组与模型组比较血清IFN-γ水平均增高,有显著性差异(P<0.01);治疗1组血清LXA4水平较其他三组明显增高,具有显著性差异(P<0.01)。结论:小儿防哮敷贴粉可以有效延长哮喘豚鼠引喘潜伏期,对哮喘豚鼠IFN-γ分泌有促进作用,且早期敷贴治疗对哮喘豚鼠LXA4释放有较强的促进作用。
Objective: To study the effect of pediatric anti-asthma powder application on the levels of IFN-γ and LXA4 in asthmatic guinea pigs. Methods: Sixty healthy guinea pigs were randomly divided into normal group, model group, treatment group, group 1 and group 2, 15 rats in each group. Normal group of natural feeding, and the remaining three groups using astaxanthin method to establish asthma guinea pig model. Treatment group 1 asthma model from the first day, given pediatric anti-asthma powder paste application, once daily, every 30min, a total of 10 days; treatment of two groups of asthma modeling, and the first 11 Day, giving pediatric anti-asthma powder paste treatment, the same treatment group 1. After the experiment, the contents of IFN-γ and LXA4 in serum were measured by ELISA. Results: Compared with the model group, the latent period of asthma in treatment group 1 and group 2 was significantly longer than that of the model group (P <0.01). The levels of serum IFN-γ in treatment group 1 and group 2 were significantly higher than those in model group (P <0.01). The level of serum LXA4 in treatment group 1 was significantly higher than that in other three groups (P <0.01). Conclusion: Pediatric anti-asthma powder can effectively prolong the latent period of asthma in asthmatic guinea pigs and promote the secretion of IFN-γ in asthmatic guinea pigs, and the early application of plaster can promote the release of LXA4 in guinea pigs with asthma.