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Objective To prepare and evaluate novel chlorine dioxide-based disinfectant powder in single-pack that is more convenient for use and iransportation.Methods Orthogonal experiment was performed to determine the recipe of the disinfectant powder.Stability test,suspension quantitative bactericidal test,simulation neld trial,and animal toxicity test were carried out to observe its bactericidal and toxicological effects.Results The orthogonal experiment showed thatthe type of water solution had no effect on the disinfectant powder and the best ratio of sodium chlorite to solid acid was 1:3.Ten grams of the disinfectant powder was fully dissolved in 20 mL water for 2 mill,and diluted to 500 mL in water.After 5-10 min,the concentration of chlorine dioxide(ClO2)solution was 266 mg/L to 276 mg/L.After stored at 54℃ for 14 d,the average concentration of ClO2 was decreased by 5.03%.Suspension quantitative bactericidal test showed that the average killing logarithm(KL)value for both Staphylococcus aureus and Escherichia coli in 100 mg/L ClO2 solution for 2 min was over 5.00.In simulation field trial,the average descending KL value for Escherichia coli in the solution containing 100 mg/L ClO2 for 5min was ovcr 3.00.The mouse acute LD50 in the solution 5 times exceeded 5000 mg/kg.The disinfectant powder was not toxic and irritativeto rabbit skin and had nomutagenic effect on mouse marrow polychrornafic erythrocytes(PCE).Conclusion The stability and bactericidal efficacy of solid chlorine dioxide-based disinfectant powder in single-pack are good.The solution containing 100mg/L ClO2 can kill vegetative forms of bacteria.The concenwation of ClO2 on the disinfecting surface of objects is 100mg/L.The disinfectant powder is not toxic and irritative.