A new seabed sediment fidelity sampler was developed and its thermal insulation performance was studied and analyzed.The temperature distribution simulation indicated that the sample quality could be insured by using this new sampler. Based on ANSYS10, the temperature finite element model of the sample cylinder was established. According to the law of conservation of energy, the unsteady heat transmit equation of the sampler under solid-liquid coupling condition was derived, then the mathematical model calculation was carried out by using a mixed finite-element finite-difference method, and two thermal insulation methods were used. The simulation was carried out by using the thickness of the thermal insulation layer and heat conductivity as the variable parameters and the temperature distribution of the sampler and related influencing factors were obtained. Optimization analysis was conducted using the simulation data and related parameters and the magnitude ranges of the parameters were obtained that could meet the design temperature requirements. The experimental data and simulation results indicated that the results were in good agreement with the realities, and this sampler might be of value for seabed sediment sampler design and manufacture.