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目的 研究持续高 G作用后是否会造成豚鼠耳石器的损伤 ,为航空航天活动中与此有关的问题提供实验依据。 方法 15只豚鼠随机分为 A、B、C3组 (n=5 ) ,A组给予 +10 Gy5 m in的刺激后 0 .5 h断头处死取双侧椭圆囊行扫描电镜观察 ,B组于 +10 Gy5 m in的刺激后 2 4h处死取双侧椭圆囊行扫描电镜观察 ,C组为对照组。处死前观察各组豚鼠的行为及前庭躯体和前庭眼动反射 ,并记录其持续时间 ,凡有任何一项症状者记为阳性。扫描电镜主要观察其囊斑表面耳石、毛细胞等的超微结构变化。 结果 +10 Gy作用 5 m in后 ,8只豚鼠出现了耳石器受损的阳性症状 ,主要表现为头位震颤 ,头位偏斜 ,身体侧滚 ,转圈 ,自发眼震和眼位偏斜等。其中以头位震颤发生率最高 ,持续时间最长。所有阳性症状均在 2 0 min内消失。扫描电镜可见 A组豚鼠的椭圆囊囊斑表面呈现两种性质的耳石改变 :1耳石消失 ,代之以大量新生的耳石前体——球状物 ;2耳石的变性融合 ,表现为多个耳石失去正常多棱型结构 ,融合为大的整体。另外 ,还可见广泛的毛细胞纤毛粘连 ,倒伏。B组豚鼠的椭圆囊囊斑表现为耳石膜明显增厚 ,高倍镜下显示表面大量新生的不成熟耳石分布 ,耳石的形状表现为两端钝圆膨大 ,呈哑铃型。同样可见广泛的毛细胞纤毛粘连和倒伏 ,程度?
Objective To study whether continuous high-G effect could cause guinea pig otolith damage and provide experimental evidence for the related problems in aerospace activities. Methods Fifteen guinea pigs were randomly divided into A, B and C3 groups (n = 5). Group A received +10 Gy5 m in. The rats were sacrificed at 24 hours after the stimulation with +10 Gy5 min, and the bilateral oval sacs were observed by scanning electron microscopy. Group C was the control group. Observed before the death of each group of guinea pigs behavior and vestibular body and vestibular eye movement reflex, and record the duration, where any one symptom was recorded as positive. Scanning electron microscopy of the main surface of the plaque surface otoliths, hair cells and other ultrastructural changes. Results The positive symptoms of otolith damage were observed in 8 guinea pigs after +10 Gy treatment for 5 mins. The main symptoms were tremor of head position, skewed head position, body roll, circling, spontaneous nystagmus and deviation of eye position . One of the highest incidence of tremor head, the longest duration. All positive symptoms disappeared within 20 min. Scanning electron microscopy shows group A guinea pig oval sac surface showed two types of otolith changes: otolith disappeared, replaced by a large number of newborn otolith precursor - ball; otolith degeneration and fusion, the performance of Multiple otoliths lose their normal polygonal structure and merge into a large one. In addition, we can see a wide range of hair cell cilia adhesion, lodging. In group B, the oval bursa of the guinea pig showed a significant thickening of the otolith, a large number of immature otoliths on the surface were observed under high magnification. The shape of the otolith was blunt bulging at both ends. The same can be seen a wide range of hair cell cilia adhesion and lodging, the extent?