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目的：研究眼镜蛇毒直接溶解因子（ＤＬＦ）对人肝癌细胞株Ｈ７４０２产生一氧化氮（ＮＯ）和脂质过氧化物（ＬＰＯ）的影响。结果：ＤＬＦ对体外培养的Ｈ７４０２细胞有杀伤作用，ＩＣ５０为２４７ｍｇ·Ｌ－１，ＤＬＦ与Ｈ７４０２细胞的生长抑制率呈量效关系。ＤＬＦ可介导Ｈ７４０２细胞产生大量ＮＯ２，ＮＯ２量与ＤＬＦ的剂量呈量效关系。在一定剂量范围内ＮＯ２量与Ｈ７４０２的生长抑制率呈显著正相关。用５７０型粘附式细胞仪测定单个细胞内ＬＰＯ，发现ＤＬＦ可使Ｈ７４０２细胞内ＬＰＯ产生增加。结论：ＤＬＦ可杀伤Ｈ７４０２细胞，其部分机制可能是通过诱导肿瘤细胞产生ＮＯ和ＬＰＯ。
OBJECTIVE: To investigate the effects of direct lytic factor (DLF) from cobra venom on the production of nitric oxide (NO) and lipid peroxides (LPO) in human hepatoma cell line H7402. RESULTS: DLF had killing effect on H7402 cells cultured in vitro with IC50 of 2.47 mg·L-1. The growth inhibitory rate of DLF and H7402 cells was dose-effect relationship. DLF can mediate a large amount of NO 2 in H7402 cells. The dose of NO 2 and DLF dose-effect relationship. There was a significant positive correlation between the amount of NO 2 and the growth inhibition rate of H7402 within a certain dose range. Using a 570-type adherent cytometer to measure LPO in a single cell, DLF was found to increase LPO production in H7402 cells. Conclusion: DLF can kill H7402 cells, and part of its mechanism may be through induction of tumor cells to produce NO and LPO.