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目的探讨雌激素对h ASCs成脂分化过程中脂滴相关特异基因DNA断裂因子相似蛋白C(The cell death-inducing DNA fragmentation 45-like effector,CIDEc)、脂滴包被蛋白(Perilipin1,Plin1)m RNA表达的影响。方法对3例患者行脂肪抽吸术,并进行h ASCs培养及传代,鉴定表面标志物;经典鸡尾酒法诱导其成脂,不同浓度(10-8、10-7、10-6mol/L)17β-雌二醇(17β-E2)作用于细胞,分别于24 h、4、7、11d收获细胞,用RT-PCR检测CIDEc、Plin1 m RNA表达情况。结果各组细胞经药物作用后,脂肪细胞中CIDEc及Plin1 m RNA的表达水平随着17β-E2浓度的升高而下降;而随着作用时间的延长,CIDEc及Plin1 m RNA表达水平分别于7 d和4 d达到高峰期,其后逐渐下降。当17β-E2浓度为10-6mol/L时,细胞中CIDEc及Plin1 m RNA的表达水平最低,差异有统计学意义(P<0.01);而加入雌激素受体阻滞剂ICI182780的一组细胞,雌激素对CIDEc及Plin1 m RNA表达的抑制作用明显减弱。结论 17β-E2能够显著降低脂肪细胞分化过程中CIDEc及Plin1的m RNA表达,并可能通过这一途径抑制脂肪细胞内脂滴的增殖。
Objective To investigate the effects of estrogen on lipid peroxidation induced by lipid peroxidation induced by lipid peroxidation in esophageal squamous cell carcinoma (h ASCs) Effect of RNA expression. Methods Three patients were subjected to liposuction and culture of h ASCs. The surface markers were identified and identified by classical cocktail method. The cells were induced to adipogenesis with different concentrations (10-8,10-7,10-6 mol / L) of 17β - estradiol (17β-E2) on the cells were harvested at 24 h, 4, 7, 11 d, respectively. The expression of CIDEc and Plin1 mRNA was detected by RT-PCR. Results The expression of CIDEc and Plin1 m RNA in adipocytes decreased with the increase of 17β-E2 concentration in each group of cells. However, the expression of CIDEc and Plin1 m RNA in adipocytes decreased with the prolongation of action time d and 4 d reached the peak, then gradually decreased. When the concentration of 17β-E2 was 10-6 mol / L, the expression of CIDEc and Plin1 m RNA in the cells was the lowest (P <0.01), while the group of cells with the ICI182780 estrogen receptor blocker , Estrogen on CIDEc and Plin1 m RNA expression was significantly weakened. Conclusion 17β-E2 can significantly reduce the mRNA expression of CIDEc and Plin1 during adipocyte differentiation and may inhibit the proliferation of lipid droplets in adipocytes through this pathway.