论文部分内容阅读
目的探讨奥曲肽对博来霉素(BLM)诱导的大鼠肺纤维化的干预作用。方法 32只Wistar大鼠随机均分为四组,A、B、C三组以气管内滴注BLM 5mg/kg建立肺纤维化模型;D组给予等体积生理盐水作为空白对照。造模后,A组皮下注射奥曲肽50μg/kg,每天2次;B组腹腔注射地塞米松1mg/kg,每天1次;连续用药21d。第21天取材,采用HE及Masson染色法观察肺组织病理变化,碱水解法测肺组织羟脯氨酸(HYP)含量,ELISA法检测支气管肺泡灌洗液(BALF)中转化生长因子β1(TGF-β1)和结缔组织生长因子(CTGF)的水平。结果与D组比较,C组大鼠肺组织炎症和纤维化明显,肺组织HYP含量和BALF中TGF-β1和CTGF表达增高;而A、B两组的上述指标均低于C组(P<0.05)。结论奥曲肽能显著减轻肺纤维化大鼠肺组织肺泡炎及肺纤维化程度;其机制可能与其降低纤维化组织TGF-β1和CTGF表达有关。
Objective To investigate the effects of octreotide on bleomycin (BLM) -induced pulmonary fibrosis in rats. Methods Thirty-two Wistar rats were randomly divided into four groups. Group A, B and C were given intratracheal instillation of BLM 5 mg / kg to establish pulmonary fibrosis model. In group D, equal volume of normal saline was given as blank control. After modeling, octreotide 50 μg / kg was injected subcutaneously in group A twice a day. In group B, dexamethasone 1 mg / kg was injected intraperitoneally once a day for 21 days. On day 21, pathological changes of lung tissue were observed by HE and Masson staining, hydroxyproline (HYP) content in lung tissue was determined by alkaline hydrolysis method, and expression of transforming growth factor-β1 (TGF-β1) in bronchoalveolar lavage fluid -β1) and connective tissue growth factor (CTGF). Results Compared with group D, the lung tissue inflammation and fibrosis in group C were significantly increased. The content of HYP in lung tissue and the expression of TGF-β1 and CTGF in BALF were increased, while those in group A and B were lower than those in group C (P < 0.05). Conclusions Octreotide can significantly reduce alveolitis and pulmonary fibrosis in lung tissue of rats with pulmonary fibrosis. The mechanism may be related to the decrease of TGF-β1 and CTGF expression in fibrosis tissue.