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cDNA-AFLP(amplified fragment length polymorphism) is used to isolate genes differentiallyexpressed in salt-stressed and unstressed wheat lines RH8706-49 and H8706-34 derivedfrom a single seed, representing a salt-stress resistant (SR) and salt-stress sensitive(SS) line, respectively. About 88.1% cDNA fragment are expressed in all the four samples,11.9% are different between the samples. 68 cDNA fragments were cloned, of which 35 weresubject to sequence analysis. Database searches indicate that 11 cDNA fragment show highhomology to known genes, which mainly include proteins related to ion transport, signaltransduction and oxidative stress. The remaining 24 cDNA show no detectable homology toknown genes, suggesting that they probably represent novel genes.
cDNA-AFLP (amplified fragment length polymorphism) is used to isolate genes differentially expressed in salt-stressed and unstressed wheat lines RH8706-49 and H8706-34 derived from a single seed, representing a salt-stress resistant (SR) and salt-stress sensitive SS) line, respectively. About 88.1% of the cDNA fragments are expressed in the four samples, 11.9% are different between the samples. 68 cDNA fragments were cloned, of which 35 weresubject to sequence analysis. to known genes, which mainly include proteins related to ion transport, signal transduction and oxidative stress. The remaining 24 cDNA show no detectable homology to known genes, suggesting that they will represent novel genes.