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目的:探讨MIF对卵巢癌血管生成的影响,术前化疗是否干扰癌细胞表达MIF。方法:选取原发上皮性卵巢癌组织蜡块58例(其中术前化疗28例)为实验组,19例正常卵巢及单纯卵巢囊肿为对照组,采用SP免疫组织化学法检测组织中MIF和CD34的表达。结果:实验组MIF表达阳性率为84.5%,与对照组(10.5%)相比差异显著(P<0.000 1)。MIF阳性癌组织的微血管密度(MVD)为(34.6±7.1)条/HP,明显高于MIF阴性组织的(20.1±6.7)条/HP及对照组的(12.0±7.5)条/HP(P值均<0.01);MIF表达强度与MVD相关(r=0.67,P<0.05)。实验组内,术前未化疗组(80.0%)与化疗组(89.3%)相比,晚期卵巢癌(88.9%)与早期卵巢癌(69.2%)相比,低分化(90.6%)与高中分化(76.9%)相比,MIF阳性率均无显著差异(P值均>0.05)。结论:MIF具有促进肿瘤组织新生血管形成的作用。化疗对癌组织MIF的表达无明显影响。
Objective: To investigate the effect of MIF on angiogenesis in ovarian cancer and whether preoperative chemotherapy can interfere with the expression of MIF in cancer cells. Methods: Fifty-eight cases of primary epithelial ovarian cancer tissues (including 28 cases of preoperative chemotherapy) were selected as experimental group, 19 cases of normal ovary and simple ovarian cyst as control group, SP immunohistochemistry was used to detect the expression of MIF and CD34 expression. Results: The positive rate of MIF in experimental group was 84.5%, which was significantly different from that in control group (10.5%) (P <0.000 1). The MVD of MIF positive cancer tissue was (34.6 ± 7.1) / HP, which was significantly higher than that of MIF negative (20.1 ± 6.7) / HP and control group (12.0 ± 7.5) / HP All <0.01). MIF expression was correlated with MVD (r = 0.67, P <0.05). Compared with the chemotherapy group (89.3%), the rate of advanced ovarian cancer (88.9%) and early stage ovarian cancer (69.2%) in the experimental group was significantly lower than that in the chemotherapy group (80.0% (76.9%), the positive rate of MIF had no significant difference (P> 0.05). Conclusion: MIF can promote neovascularization of tumor tissue. Chemotherapy had no significant effect on the expression of MIF in cancer tissues.