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通过免疫组织化学的方法，观察血小板源性生长因子（PDGF）对于培养脐静脉内皮细胞血管内皮细胞生长因子（VEGF）水平的影响，揭示PDGF促进新生血管形成的机制。实验结果：缺氧培养时内皮细胞胞浆VEGF水平上升；不同剂量的PDGF在常规或缺氧培养条件下均能增加内皮细胞VEGF水平，且呈剂量依赖性，提示PDGF可能通过直接促血管形成生长因子的介导作用间接促进新生血管的形成。
The effect of platelet-derived growth factor (PDGF) on the level of vascular endothelial growth factor (VEGF) in cultured human umbilical vein endothelial cells was observed by immunohistochemistry to reveal the mechanism by which PDGF promotes neovascularization. The results showed that the level of VEGF in endothelial cells increased in hypoxia culture. Different doses of PDGF could increase the VEGF level in endothelial cells under normal or hypoxia conditions in a dose-dependent manner, suggesting that PDGF might directly induce angiogenesis The mediation of factors indirectly promote the formation of new blood vessels.