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Based on the self-organizing modeling principle in the dissipative structure theory, the paper regards the migration coupling between the regions as the influence factor to create the model of regional urbanization simulation and prediction, which is more practical to simulate the space-time and dynamic evolvement characteristics of regional urbanization level. According to the historical data about the population and urbanization of Jiuquan, Jiayuguan and Yumen cities, the past 50 years dynamic evolvement trend is simulated and the following 50 years dynamic evolvement trend is predicted with the urbanization dynamic evolvement model based on the self-organizing model. The analysis of effective factors such as economic increase, resources exploitation, ecosystem construction,traffic location, national policies, population migration are given, then the reasons of the dynamic evolvement differences of the three cities urbanization are drawn.The study suggests that the self-organizing model is fit for the simulation of Jiuquan-Jiayuguan-Yumen regional urbanization level in the past 50 years, and the departure between the stimulant and the actual data is under 5%, so it can be used to predict the urbanization future of the three cities. The predicted results show that the regional population will reach 1.074 million and the regional urbanization level will be 72.56%.