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血管紧张素转换酶2(ACE2)为肾脏水盐代谢的重要调控因子,其通过调节肾脏局部血流量影响肾脏功能。为了证明爬行动物是否存在ACE2基因,本研究以中华鳖作为实验材料,应用RT-PCR方法扩增并克隆ACE2基因。结果表明,克隆的这段中华鳖ACE2序列长度为355 bp,与人、鸡和斑马鱼ACE2核苷酸序列的同源性分别达到70.3%、80%和65.1%,推导的氨基酸序列同源性分别为63.8%、79.3%和56.9%。表明中华鳖肾脏ACE2与鸡、人的ACE2均有较高的同源性,其中,与鸡的同源性最高。通过遗传进化分析发现,中华鳖肾脏ACE2与鸟类亲缘关系最近,符合物种进化理论。
Angiotensin-converting enzyme 2 (ACE2) is an important regulator of water and salt metabolism in the kidneys, which affects renal function by regulating local blood flow in the kidney. In order to prove that ACE2 gene exists in reptiles, we used Chinese softshell turtle as experimental material and amplified and cloned ACE2 gene by RT-PCR. The results showed that the length of ACE2 gene was 355 bp and the nucleotide sequence of ACE2 in human, chicken and zebrafish was 70.3%, 80% and 65.1%, respectively. The deduced amino acid sequence homology Respectively 63.8%, 79.3% and 56.9%. The results showed that ACE2 in kidney of Chinese soft-shelled turtle had higher homology with that of chicken and human ACE2, of which the homology with chicken was the highest. Genetic evolution analysis revealed that the kidney ACE2 of Trionyx sinensis has the closest genetic relationship with birds and conforms to the theory of species evolution.