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运用酸性聚丙烯酰胺凝胶电泳(A-PAGE)和十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)对偃麦草属(Elytrigia)24个物种的醇溶蛋白和谷蛋白进行了研究。以中国春为参照,按醇溶蛋白和谷蛋白电泳图谱条带迁移距离大小和条带多态性对供试材料进行聚类分析。结果显示,24份供试材料均呈现不同的醇溶蛋白和谷蛋白电泳图谱,共分离出醇溶蛋白带纹83条和谷蛋白带纹53条,多态性均达100%,并且在相同染色体组组成的物种中,染色体数目越多,其蛋白带纹越多;偃麦草属24个物种的醇溶蛋白和谷蛋白图谱均有明显差异,蛋白图谱可作为鉴定偃麦草属物种的指纹图谱。聚类分析结果显示,在材料间的醇溶蛋白遗传相似性系数为0.66时,24份材料被划分为6个主要类群,含E和St基因组的物种具有较近的亲缘关系;在谷蛋白遗传相似性系数为0.62时,24份材料被聚为4大类。聚类结果表明,染色体组成未知的物种Et.pachynera可能为异源多倍体物种。
The gliadin and glutelin of 24 species of Elytrigia were studied using acidic polyacrylamide gel electrophoresis (A-PAGE) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) the study. Based on the Chinese spring, the cluster analysis was conducted on the tested materials according to the migration distance and band polymorphism of gliadin and gluten electrophoresis bands. The results showed that all of the 24 tested materials showed different prolamin and gluten electrophoresis patterns, and 83 prolamin bands and 53 gluten bands were isolated and the polymorphisms were all 100% Genome chromosome composition of the species, the more chromosomes, the more protein bands; 24 species of Elymus gliadin and gluten profiles were significantly different, protein map can be used as a fingerprint identification of the genus Agropyron species . Cluster analysis showed that when the genetic similarity coefficient of gliadin between materials was 0.66, 24 materials were divided into 6 major groups, and the species with E and St genomes had closer genetic relationship; in gluten inheritance When the similarity coefficient was 0.62, 24 materials were grouped into four categories. The clustering results showed that Et.pachynera, an unknown species of chromosome, may be a heterologous polyploid species.