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目的:应用cDNA芯片技术研究食管鳞癌的异常基因表达,并对这些基因的功能进行初步分析。方法:选择382个肿瘤相关基因克隆,制备成cDNA芯片,提取食管鳞癌组织以及相应正常食管组织RNA,反转录后标记为cDNA探针,与自行制备的cDNA芯片杂交,经扫描及分析后筛选出两种组织中差异表达基因。结果:发现差异表达基因75个,表达上调基因44个,下调基因31个,包括癌基因、抑癌基因、细胞周期相关蛋白、粘附因子、基质金属蛋白酶、信号传导因子、生长因子及其受体、与细胞代谢相关的酶等。结论:食管鳞癌的发生发展涉及多基因改变,cDNA芯片技术是疾病基因筛选的有效方法。
OBJECTIVE: To study the abnormal gene expression in esophageal squamous cell carcinoma by cDNA microarray and to analyze the function of these genes. METHODS: A total of 382 tumor-associated genes were cloned and sequenced. CDNAs were prepared and RNA extracted from esophageal squamous cell carcinoma and normal esophageal tissues was reverse transcribed and labeled with cDNA probes. The cDNAs were hybridized with cDNA prepared by ourselves. After scanning and analyzing Two differentially expressed genes were screened out. RESULTS: There were 75 differentially expressed genes, 44 genes were up-regulated, and 31 genes were down-regulated, including oncogenes, tumor suppressor genes, cell cycle related proteins, adhesion factors, matrix metalloproteinases, signal transduction factors, growth factors and their receptors Body, enzymes related to cell metabolism and the like. Conclusion: The occurrence and development of esophageal squamous cell carcinoma involve multiple gene changes. CDNA microarray is an effective method to screen disease genes.