论文部分内容阅读
目的研究光敏剂琥红(RB)、赤鲜红B、α-三噻吩对实验室饲养及野外白纹伊蚊4龄幼虫的杀灭效应。方法分别在实验室和野外条件下,计数不同药物浓度、不同光源照射和照射强度、不同处理时间的RB、赤鲜红B、α-三噻吩作用后白纹伊蚊4龄幼虫的死亡数,分析其影响杀蚊幼虫的实验参数。用组织化学的方法,研究其毒理机制。结果RB、赤鲜红B、α-三噻吩无暗毒性。用固定光源100W日光灯照射,照射强度为320×102lx,时间为6h;RB药物浓度分别为10、25μg/ml,赤鲜红B药物浓度分别为100、150μg/ml,皆可达到100%杀灭白纹伊蚊4龄幼虫的效果。用中低强度的太阳光照射5h,α-三噻吩药物浓度为1μg/ml,亦可达到100%杀灭白纹伊蚊4龄幼虫的效果。初步将赤鲜红B、α-三噻吩分别应用于野外不清澈和清澈自然水体内控制白纹伊蚊幼虫,赤鲜红B、α-三噻吩在野外不清澈水体内的杀虫率分别为46%和49%,而在清澈自然水体内的杀虫率分别为67%和89%;但当持续强度太阳照射,α-三噻吩在前2种水体内杀虫率皆可达到98%。组织化学试验结果显示:用RB、赤鲜红B、α-三噻吩处理的白纹伊蚊4龄幼虫,经光毒作用后,蚊中肠绒毛消失,细胞肿胀,细胞核消失。脂肪体在表皮下广泛分布,马氏管外形不规则及管腔狭窄等病理变化。结论RB、赤鲜红B、α-三噻吩3种光敏剂皆是高效、实用的光敏化杀虫剂。
Objective To study the killing effect of photosensitizer RB, erythrosine B and α-trithiophene on 4th instar larvae of laboratory and field. Methods The numbers of deaths of 4th instar larvae of Aedes albopictus after RB, Akabane B, and α-thiophene treatments with different drug concentrations, different light sources and intensities, and different treatment time were counted under laboratory and field conditions, respectively Its impact on mosquito larvae experimental parameters. Using histochemical methods to study its toxicological mechanism. Results RB, red red B, α-thiophene no dark toxicity. With a fixed light source 100W fluorescent light irradiation intensity 320 × 102lx, time 6h; RB drug concentrations were 10,25μg / ml, Akagi B drug concentrations were 100,150μg / ml, can reach 100% kill white Aedes albopictus 4th instar larvae effect. Low and medium intensity sunlight was applied for 5h, the concentration of α-tnithiophene was 1μg / ml, and the effect of killing 100% of 4th instar larvae of Aedes albopictus could also be achieved. Preliminary application of the red bright red B and α-trithiophene to the control of Aedes albopictus larvae in clear and clear natural water in the wild respectively showed that the insecticidal rates of Chek B, α-trithiophene in the clear water were 46% And 49% respectively, while the insecticidal rates in clear and natural water were 67% and 89%, respectively. However, the insecticidal rate of α-t-thiophene in the first two kinds of water could reach 98% when the intensity of solar radiation was constant. Histochemical tests showed that the 4th instar larvae of Aedes albopictus treated with RB, Akabane B, and α-thiophene showed disappearance of villi, swelling of cells and disappearance of nuclei. Fat body is widely distributed in the epidermis, irregular appearance of Markov tube and stenosis and other pathological changes. Conclusions The three photosensitizers RB, Akabane B, and α-terthiophene are effective and practical photoperiod insecticides.