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目的:建立痰咳净片中桔梗皂苷D的含量测定方法。方法:采用高效液相色谱法-蒸发光散射检测器(HPLC-ELSD),色谱柱为Kromasil C18(4.6 mm×250 mm,5μm),流动相为乙腈-水(26∶74),流速为1.0 m L/min,ELSD漂移管温度为80℃,气体流速为2.6 SLPM/min。结果:桔梗皂苷D进样量在0.499 4~12.485 4μg线性范围内峰面积线性关系良好(r=0.999 7),平均回收率98.51%,RSD=0.8%(n=6)。结论:该方法准确、可靠,重复性好,灵敏度高,可用于痰咳净片的质量控制标准。
Objective: To establish a method for the determination of platycodin D in Tanke net. Methods: HPLC-ELSD was used. The chromatographic column was Kromasil C18 (4.6 mm × 250 mm, 5 μm). The mobile phase was acetonitrile-water (26:74) and the flow rate was 1.0 m L / min, the ELSD drift tube temperature was 80 ° C, and the gas flow rate was 2.6 SLPM / min. Results: The peak area of Platycodin D in the linear range of 0.499 4 ~ 12.485 4 μg was linear (r = 0.999 7) with the average recovery of 98.51% and RSD 0.8% (n = 6). Conclusion: The method is accurate, reliable, reproducible and sensitive. It can be used as a quality control standard for Tanke tablets.