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本实验用还原型尼克酰胺腺嘌呤二核苷酸脱氢酶组织化学方法结合GFAP免疫组化方法,对微量海人酸皮质内注射后的一氧化氮合酶阳性神经元和胶质细胞等的变化进行了研究.结果发现:注射区内的一氧化氮合欧阳性神经元很快消失;注射侧皮质和海马的旧阳性神经元和神经末梢溃变;至注射海人酸后8h,这些变化波及到对侧皮质和海马.随着皮质内一氧化氮合酶阳性神经元的溃变,在注射侧皮质和海马内的一些神经元、星形胶质细胞出现新的一氧化氮合酶活性,这些新的酶活性可能是诱导型的一氧化氮合酶.这些结果表明:表达一氧化氮合酶的皮质和海马神经元对海人酸的毒性很敏感,神经细胞和非神经细胞中出现的诱导型的一氧化氮合酶可能是海人酸引起脑损伤时机体的适应性反应.
In this study, the use of reduced nicotinamide adenine dinucleotide dehydrogenase histochemical method combined with GFAP immunohistochemical method of trace kainic acid cortical injection of nitric oxide synthase positive neurons and glial cells Changes were studied. The results showed that the NO-positive neurons in the injection area disappeared quickly; the old positive neurons and nerve endings in the cortex and hippocampus of the injected side degenerated; these changes reached the contralateral cortex And the sea horse. With the degeneration of cortical nitric oxide synthase positive neurons, some of the neurons in the injected cortex and hippocampus showed novel nitric oxide synthase activity astrocytes, which may be Inducible nitric oxide synthase. These results indicate that cortical and hippocampal neurons expressing nitric oxide synthase are sensitive to the toxicity of kainate and that inducible nitric oxide synthase found in neurons and non-neuronal cells may be brain damage caused by kainate The body’s adaptive response.